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pkan aid 9myc  (Addgene inc)


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    Structured Review

    Addgene inc pkan aid 9myc
    Pkan Aid 9myc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pkan+aid+9myc/pKan-AID*-9myc+(Plasmid+%2399522)/pmc12006795-57-16-17
    Average 92 stars, based on 5 article reviews
    pkan aid 9myc - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Sequencing:

    Article Title: Nutrient starvation-induced Hda1C rewiring: coordinated regulation of transcription and translation.
    Article Snippet: .. The TRP1 - p GAL1 cassette and AID sequence were amplified with pFA6a-TRP1-pGAL1-3HA (Addgene plasmid #41609) and pKan-AID*-9myc (Addgene plasmid #99522), respectively by using primers that created Sal I sites. ..

    Article Title: Nutrient starvation-induced Hda1C rewiring: coordinated regulation of transcription and translation
    Article Snippet: .. The TRP1 -p GAL1 cassette and AID sequence were amplified with pFA6a-TRP1-pGAL1-3HA (Addgene plasmid #41609) and pKan-AID*-9myc (Addgene plasmid #99522), respectively by using primers that created Sal I sites. ..

    Amplification:

    Article Title: Nutrient starvation-induced Hda1C rewiring: coordinated regulation of transcription and translation.
    Article Snippet: .. The TRP1 - p GAL1 cassette and AID sequence were amplified with pFA6a-TRP1-pGAL1-3HA (Addgene plasmid #41609) and pKan-AID*-9myc (Addgene plasmid #99522), respectively by using primers that created Sal I sites. ..

    Article Title: Nutrient starvation-induced Hda1C rewiring: coordinated regulation of transcription and translation
    Article Snippet: .. The TRP1 -p GAL1 cassette and AID sequence were amplified with pFA6a-TRP1-pGAL1-3HA (Addgene plasmid #41609) and pKan-AID*-9myc (Addgene plasmid #99522), respectively by using primers that created Sal I sites. ..

    Article Title: Bidirectional roles of the Ccr4-Not complex in regulating autophagy before and after nitrogen starvation.
    Article Snippet: Macroautophagy/autophagy is a highly conserved catabolic process by which cytoplasmic constituents are delivered to the vacuole/lysosome for degradation and recycling.. To maintain cellular homeostasis and prevent pathologies, the induction and amplitude of autophagy activity are finely controlled through regulation of ATG gene expression.. Here we report that the Ccr4-Not complex in Saccharomyces cerevisiae has bidirectional roles in regulating autophagy before and after nutrient deprivation.

    Plasmid Preparation:

    Article Title: Nutrient starvation-induced Hda1C rewiring: coordinated regulation of transcription and translation.
    Article Snippet: .. The TRP1 - p GAL1 cassette and AID sequence were amplified with pFA6a-TRP1-pGAL1-3HA (Addgene plasmid #41609) and pKan-AID*-9myc (Addgene plasmid #99522), respectively by using primers that created Sal I sites. ..

    Article Title: Nutrient starvation-induced Hda1C rewiring: coordinated regulation of transcription and translation
    Article Snippet: .. The TRP1 -p GAL1 cassette and AID sequence were amplified with pFA6a-TRP1-pGAL1-3HA (Addgene plasmid #41609) and pKan-AID*-9myc (Addgene plasmid #99522), respectively by using primers that created Sal I sites. ..

    Article Title: A compendium of chromatin contact maps reflecting regulation by chromatin remodelers in budding yeast
    Article Snippet: .. In SPT6-AID and CHD1ISW1-AID strain, AID tagging of endogenous genes were performed by polymerase chain reaction (PCR) products with pKan-AID*-9myc plasmid. pKan-AID*-9myc was a gift from Helle Ulrich (Addgene plasmid # 99522 ; RRID:Addgene_99522). ..

    Polymerase Chain Reaction:

    Article Title: A compendium of chromatin contact maps reflecting regulation by chromatin remodelers in budding yeast
    Article Snippet: .. In SPT6-AID and CHD1ISW1-AID strain, AID tagging of endogenous genes were performed by polymerase chain reaction (PCR) products with pKan-AID*-9myc plasmid. pKan-AID*-9myc was a gift from Helle Ulrich (Addgene plasmid # 99522 ; RRID:Addgene_99522). ..

    Homologous Recombination:

    Article Title: Bidirectional roles of the Ccr4-Not complex in regulating autophagy before and after nitrogen starvation.
    Article Snippet: Macroautophagy/autophagy is a highly conserved catabolic process by which cytoplasmic constituents are delivered to the vacuole/lysosome for degradation and recycling.. To maintain cellular homeostasis and prevent pathologies, the induction and amplitude of autophagy activity are finely controlled through regulation of ATG gene expression.. Here we report that the Ccr4-Not complex in Saccharomyces cerevisiae has bidirectional roles in regulating autophagy before and after nutrient deprivation.



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    Image Search Results


    Journal: microPublication Biology

    Article Title: The CRISPR/Cas9 system forms a condensate in the yeast nucleus

    doi: 10.17912/micropub.biology.001039

    Figure Lengend Snippet:

    Article Snippet: The AID* sequence, obtained from the plasmid pKan-AID*-9myc (addgene code #99522; #2188 in the Urlich collection) , was converted into a 4a module by PCR, whereas an AID* 3a module was ordered as a synthetic gene (gBlocks HiFi Gene fragments, IDT).

    Techniques: